Integrated Dna Purification and Amplification Using Fta

This paper reports the development of a combined microfluidic system for purification and amplification of DNA from FTA ® paper. Using FTA ® paper eradicates the need to elute nucleic acids from a solid-phase matrix prior to downstream processing, enabling both purification and amplification to be easily performed in a single chamber on the microfluidic device. Following optimization of the methodology and associated control systems, successful amplification of the D21 S11 locus from human buccal swab samples stored on FTA ® paper was achieved. Such a system offers advantages in terms of a simple sample introduction interface and the ability to process archived samples in an integrated microfluidic environment with minimal risk of contamination.